【摘要】 <正> 自从Grace(1962)成功地从昆虫中建立了第一个细胞株后,到1975年已从52种昆虫中分离到115个细胞株(Maramorosch,1975)。培养的细胞株被广泛地应用于病毒学、发育生物学、细胞生物学、病理学、毒物学、生物化学和遗传学等方面的研究(Hink,1972;Weiss,1971;Vago,1971和 1972;Kurstak和Maramorosch,1976)。 为了进一步弄清昆虫病毒和寄主细胞间的相互关系及环境对它们的影响,我们在离体条件下培养了鳞翅目民虫的血球细胞和卵巢细胞。现把初步结果简述于下。更多还原

【Abstract】 The authors used Grace’s medium supplemented with 20% fetal calf serum, 2% Philosamia cynthia ricini hemolymph and vitamins E, K, B13, B12 (each 0.001 mg/ 100ml) and C (1mg/100ml) for the culture of cells from three lepidopterous insects, and succeeded in maintaining the prolonged survival and growth of cells in vitro.Hemocytes of Philosamia cynthia ricini and Euproctis similis survived for 211 days and 91 days, respectively.Hemocytes of Mythimna separata subcultured after growth for four months, and in serial passage totally for 15 times (survived for 15 months).Cells derived from both Philosamia cynthia ricini and Mythimna separata ovarian tissue survived for 7 months and 43 days, respectively.Experimental results show that the Grace’s medium can support the above mentioned cells, growth and survival in vitro for a long period.更多还原