【摘要】 <正> 胃泌素(Gastrin,又称胃素或促胃液素)是胃窦和小肠上部G细胞分泌的一种重要的胃肠激素。早在1906年,Edkins就为它命了名,但对它的本质及其是否存在经历了近60年的反复争论。1964年Gregory和Tracy第一次把它提纯,并进而阐明化学结构和人工合成,这才确定了它是一种真正的激素。胃泌素的分子结构有多种形式,其中最通常而生物活性最强的形式是由17个氨基酸组成的小胃泌素(简称G17)。G17的存在又有两种类型:在第12位的酪氨酸有硫酸酯基团的称为胃泌素Ⅱ,没有此基团的称为更多还原

【Abstract】 A radioimmunoassay (RIA) of gastrin was established for the first time in this country by using a high-quality anti-serum, higher specific activity of 125 1-SHG-1 and the double antiserum separation technique. Antibodies against gastrin have been elicited in rabbits which were immunized by multiple-site intradermal and foot pad injection with purified gastrin-17 conjugated to bovine serum albumin. The antiserum thus produced had a higher potency and specificity. The sensitivity of this RIA was 5pg, the precision, 2.79% and the recovery rate, 90-108%. Gastrin value showed a linear decrease concomitant with the dilution of the serum from 1:1 to 1:8. The fasting gastrin levels of 79 healthy individuals ranged from 11 pg per ml to 140 pg per ml, with a mean of 62.12±28.71 pg per ml (mean±SD). Synthetic human gastrin-I-17 and natural gastrin 1 were kindly supplied by Professor P. A. Gregory of Liverpool, England and Professor M. I. Grossman of Los Angeles, USA.更多还原