【摘要】 本文报道了口服醋酸棉酚(4毫克/公斤/日)两年的成年雄性恒河猴3只及对照猴2只的心肌超微结构、心肌 LDH 和 SDH 的电镜组化的观察,初步结果如下:1.猴心肌肌原纤维Ⅰ带 Z 线两侧各出现一条由电子密度较大的颗粒组成的细线,称 N线。服棉酚后 N 线的电子密度较对照组为低,同时Ⅰ带的宽度相应加大,提示棉酚有加深心肌纤维松弛状态的可能性。其它超微结构的改变如溶酶体数量增多,内质网扩张,局部地区的线粒体异常堆集、排列紊乱、数量明显增多,部分线粒体呈现轻度肿胀。2.组化方面,定位于心肌细胞线粒体和内质网中与心肌无氧糖酵解有关的 LDH 以及定位于线粒体的 SDH 活性均于服棉酚后增强。我们初步分析和讨论了以上结果与心肌纤维的松弛和收缩可能有关。更多还原

【Abstract】 The ultrastructural and histochemical changes of cardiac muscles in three adult male Rhesus monkeys,administered orally of gossypol acetic acid of a daily dose of 4 mg/kg for 2 years were studied with two control animals by means of electron microscopic techniques. The results obtained are as follows: 1.It was shown that a fine line,the so-called N line,characterized by its high electron density,was observable at both sides of the Z line in I-band of monkey cardiac myofibrilla.The electronic density of the N line became less intensive,and the I-band increased in breadth following gossypol treatment,it suggested that gossypol might induce muscle relaxation and thus inhibit contraction.Other ultrastruetural changes such as increase in amount of lysosomes,distension of endoplasmic reticulum, local accumulation,derangement and swelling of mitochondria in myocardiac fibers of treated animals were also evident. 2.Histochemically,there was an increase in enzyme activity of LDH(an enzyme concerned with anaerobic glycolysis)in mitochondria and endoplamic reticulum,and SDH(an enzyme related to cellular oxidation)in mitochondria of cardiac muscles after gossypol treatment.Their possible relation to muscle relaxation and contraction were analyzed and discussed.更多还原