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LARGE-SCALE PURIFICATION OF PROTHORACICOTROPIC HORMONE OF THE SILKWORM (BOMBYX MORI)
【摘要】 <正> Prothoracicotropic hormone has been successfully extracted from as many as 2,800,000 silkworm male adult heads and purified partially through an 8-step procedure. Approximately 7μg of the crude preparation ("crude PTTH") shows activity in the debrained Samia pupa test. A further 4-step chromatographic purification of the "crude PTTH" derived from 100,000 Bombyx heads yields 3.4mg of purified preparation ("highly purified PTTH"), of which 7ng causes adult development in a debrained Samia pupa.
【Abstract】 Prothoracicotropic hormone has been successfully extracted from as many as 2,800,000 silkworm male adult heads and purified partially through an 8-step procedure. Approximately 7μg of the crude preparation ("crude PTTH") shows activity in the debrained Samia pupa test. A further 4-step chromatographic purification of the "crude PTTH" derived from 100,000 Bombyx heads yields 3.4mg of purified preparation ("highly purified PTTH"), of which 7ng causes adult development in a debrained Samia pupa.
- 【文献出处】 Science in China,Ser.A ,中国科学A辑(英文版) , 编辑部邮箱 ,1980年08期
- 【被引频次】2
- 【下载频次】27