【摘要】 <正> 由红藻提取的琼胶、卡拉胶等多糖胶质,已被广泛应用于食品、医药、轻工和科研等方面。这些多糖中最主要的化学成分是3.6—内醚半乳糖和半乳糖。要深入研究红藻类的生化过程及所含多糖的理化性质,准确分析红藻多糖中这两种主要单糖的含量,就成为十分必要的了。为此,就要求建立一个准确而简便的分析方法。更多还原

【Abstract】 This method may be applied to the determination 3.6-anhydrogalactoseand galactose in red algal polysaccharides. The 3.6-anhydrogalactose wasdetermined directly with the resorcinal reagent, using fructose as a refer-ence sugar. The anthrone reagent was used for the simultaneous determ-ination of 3.6-anhydrogalactose and galactose was obtained by difference.The results obtained in the investigation were summarized as follows: 1. Resorcinal reagent was used for the determination of 3.6-anhydr-ogalactose. The absorbance reached a maximum value after 10min at 80℃.The absorption spectrum had maximum absorbance at 550nm. The absorbancewas measured within 15min. The reaction was carried out in diffuse sincethe color fades on exposure to sunlight. 2. Anthrone reagent was used for the simultaneous determination ofthe two sugar. The absorbance reached a maximum value after 20min at80℃. The absorption spectrum had maximum absorbance at 640nm. 3. The results in Table I showed that concentration of 3.6-anhydr-ogalactose and galactose in red algal polysacchardes. The relative averagedeviation of the method was below 1%. The vesults in Table Ⅱ showedthat the recovery of 3.6-anhydrogalactose was 98%-to 100% with r-esorcinal reagent. The recovery of galactose was 98% to 100% with anth-rone reagent.更多还原