【摘要】 本文介绍了粒系定向干细胞的体内扩散盒琼脂培养技术。将含0.5％琼脂的骨髓细胞悬液注入扩散盒,植入正常小鼠腹腔培养5天,细胞团生长良好。骨髓细胞种植量与细胞团产率之间呈正相关。体内扩散盒琼脂培养法无需提供外源性CSF,造血干细胞能在更接近干体内正常内环境的条件下增殖,是反映粒系定向干细胞相对数量的一个较好的实验技术。更多还原

【Abstract】 This paper describes a modified diffusion chamber culture technique originally introduced by Gordon in 1974 for the quantitation of granulocytic committed precursor cells. Adult mice have been used as donors and hosts. Molten agar is added to the 1640 medium, which contains the required number of nucleated bone marrow cells for assay, to make a final agar concentration of 0.5%. 200μl of this mixture is injected into each chamber. After five days incubation, colonies are scored. The experimental data show the linear relationship between the number of nucleated bone marrow cells per chamber and of colonies scored. This culture system therefore provides a convenient and reliable clonal assay for CFU-C. and in contrast to the agar colony assay in vitro, does not require an exogenous source of CSF. The main modification in our experiments is that the final agar concentration is 0.5% instead of 0.3% and the days for incubation are shortened to five.更多还原