【摘要】 将球虫卵囊悬浮液少许混入较大量的2％重铬酸钾溶液中,于室温下静置4—7天,这样卵囊就会孢子化。用吸管吸取一滴含此种孢子化卵囊的溶液于载玻片上,加上一滴等渗氯化钠溶液稀释至每个视野含1—2个卵囊。在低倍显微镜下,用玻璃毛细吸管对着一个卵囊,将其吸取,然后吹落于铺有琼脂薄膜的玻片上,并在镜下检查证实只有一个卵囊时,用细镊子划出含此卵囊的相应部分琼脂薄膜,并喂给一天龄小鸡。这些试验小鸡应注意保温和分别隔离饲养,喂以煮沸过的饲料和饮水,避免一切环境污染的可能性。经过排卵囊前期之后,这些小鸡就应在粪便中排出单纯一个种的卵囊。更多还原

【Abstract】 A small volume of coccidial oocyst suspension is mixed with ample quantity of 2 % potassium dichromate solution. After standing in room temperature for 4-7 days, most of the oocysts will sporulate. By using a pipette, a drop of this solution containing sporulated oocysts is drawn in and then blown onto a slide, while examining under a low power microscope, isotonic sodium chloride solution is added to dilute it to reach sush a point with only 1-2 oocysts per microscopic field . Then,the manipulater must establish his own skill to use a capillary pipette to such in a single oocyst and then transfer it onto another slide coated with agar gel film. At this time, it is important to make certain that there is only one oocyst on the film. Finally the film supporting the oocyst is cut and picked up with a pair of fine forceps to feed a day -old chick. All such experimental chicks must be reared individually in separate cases and supplied with well cooked feeds and boiled water to avoid environmental contamination of coccidial oocysts. Such chicks will excrete oocysts of a single species of coccidia after the preookinesis period.更多还原