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~3H-TdR淋巴细胞转化试验中制样方法的探讨

Experiment of lymphocyte transformation with ~3H-TdR——A study of method of sample preparation

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【作者】 杨希震; 吴佳平; 何荣霞; 沈亚瑛; 徐鑫川; 张满达;

【Author】 Yang Xi-zhen Wu Jia-ping He Rong-xiaShen Ya-ying Xu Xin-chuan Zhang Man-da(Jiangsu Institute of Nuclear Medicine,

【机构】 江苏省原子医学研究所; 江苏省原子医学研究所;

【摘要】 <正> 淋巴细胞转化反应是体外测定机体免疫功能的常用方法,但在采用~3H-TdR参入的淋巴细胞转化试验中,制样方法的不同,对结果的正确性影响很大。本文就消化法与滤膜法作了比较试验,还对不同规格的滤膜进行了筛选,现将结果报道如下。

【Abstract】 During the experiment of lymphocyte transformation with 3H-TdR, we found the counting rate of preparing sample using filter method was higher than that using chemical-digest method. Experiment showed the counting rate for 2 μCi was doubled in filter method than digest method.Moreover, when homemade 49 glass fiber disc was used in the filter method, the counting rate was markedly increased, in comparing with 0.8μ, 3μ, 5μ millipore filter The filtering rate was quite rapid and gave reproducible results. The liquid scintilla-tor after filter disc being removed could be used over again.

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