【摘要】 红宝石脉冲激光微光束仪用于生物活细胞的微照射和解剖.本文详细分析和讨论了仪器总体设计的特点.论述激光微光束的理论计算.为了获得小的聚焦光斑直径和高的聚焦效率,较佳的折衷方案是选择低倍的显微镜目镜和高倍大数值孔径的显微镜物镜.而且,为了获得小的聚焦光斑尺寸,物镜必须严格校正球差,并且激光器应是基模运转的.文中还详细讨论了减少激光束通过显微镜光能量损失的方法,以提高聚焦系统的效率.更多还原

【Abstract】 A ruby pulsed laser microbeam instrument was applied to the microirradiation and dissection of the living cell. In this paper, the features of integral design of instrument are analysed and are discussed in detail. Theoretical calculation of laser microbeam is described. For obtaining small focusing spot diameter and high focusing efficiency, the better compromise is to choose the microscope eyepiece of low magnification and the microscope objective of high magnification with big numerical aperture. Furthermore, microscope objective must exactly correct spherical aberration and laser is desired the operation with fundamental mode for obtaining small focusing spot size. In this paper, the method of decreasing loss of light energy for laser beam through microscope is discussed in detail, so as to increase efficiency of the focusing system.更多还原