【摘要】 <正> 顺二氨二氯铂[Ⅱ]（Cis-[Pt（NH₃）₂Cl₂]）（以下简称顺氨铂）是无机重金属抗肿瘤新药。文献报道顺氨铂能抑制若干种动物肿瘤,如小鼠S₁₈₀、艾氏腹水癌、网织细胞瘤、L₁₂₁₀白血病、大鼠W₂₅₆癌肉瘤、Dunning腹水型白血病、二甲苯蒽（DMBA）诱发的乳腺瘤以及腹水型B-16黑色素肉瘤的生长。临床试用发现顺氨铂对恶性淋巴瘤、软组织肉瘤、睾丸肿瘤、鼻咽癌、肺癌等也有不同程度的化疗作用。对培养的癌瘤细胞和正常细胞（鸡成纤维细胞）都有明显的细胞毒效应。实验研究证明,顺氨铂抗瘤作用的机制主要是能选择性地抑制DNA的合成。这种抑制作用的形态学表现是有丝分裂活性降低、细胞退化、坏死。这些细胞水平的损伤可能是引起肿瘤消退的更多还原

【Abstract】 The effects of Gis--DDP, an inorganic metal antitumor agent, on the BEL--7402 cellsin vitro was studied. After cis--DDP treatment of the BEL--7402 cells for 2-6 h, the mito-tic activity was inhibited. After 12h of treatment, this effect became more pronounced andno mitosis could be observed. Gis--DDP induced cytological anormalies such as nucleolar bli-stering, chromatin accumulation and the decrement of cytoplasm. After 24h, necroses werefound in some BEL-7402 cells. Thus the percentage of survival cell fraction reducedmarkedly.更多还原