【摘要】 <正> 5-羟色胺(5-HT)是参与体内许多生理活动调节过程的重要神经介质。由于5-HT必须通过受体才能发挥其生理作用,因此,5-HT受体亲和力的测定,已经成为近年来研究神经生理、神经药理学的一个不可缺少的方法。 目前测定脑内5-羟色胺受体亲合力的方法很多。我们主要依据Perontka和Snyder(1979)方法,初步建立了脑内5-HT受体亲和力的测定方法,现简要报告如下:更多还原

【Abstract】 This article indroduces the method of affinity binding of ~3H--5--hydroxytryptamine(~3H-5--HT) to rat brain serotonin receptor in vitro. erebral binding of ~3H-5-HT was 95.1±4.22pci/mg protein, but cerebellerbinding of ~3H-5-HT was only 45. 3±3. 22 pci/mg protein (Specific: nonspecific ratio=2.1:1). ~8H--5--HT binding to serotonin receptor can be competed with unlabelled agonist (5HT)or antagonist (cinaserin)of serotonin receptor. There was a gradual reduction in the ~3H-5HT binding in association with the increaseof the agonist and antagonist of seretonin receptor.更多还原