【摘要】 <正> 我国胆石病以原发性胆管结石多见,无论是用手术或中西医结合排石疗法均不易彻底清除干净。因此,我们想从探讨结石的成因入手,进而寻找有效的溶石和防石药物,从1963年开始对胆石的成分和结构进行了化学定量分析和组织化学的研究,现报告如下。更多还原

【Abstract】 Quantitative analysis of gallstones in 65 patients revealed that they were composed ofcholesterol, bilirubin, calcium, phosphates and nitrogen in the insoluble residua. 38 caseswere pigment stones, whereas 27 cases were mainly cholesterol in nature. 28 out of 30cases of primary cholangiolithiasis are pigment stones. In pigment stones, less than 50% by weight of the constituents can be detected byquantitative analysis. In order to find out the undetected components, histochemical studyof gallstone was carried out. A lot of substance giving a positive staining to Schiff’s peroidicacid (PAS) could be found after bilirubin was removed, and only small amount presentbefore that procedure. The distribution and density of PAS stained substances run parallelwith amount of bilirubin. It is presumed that the PAS stain positive substances wereundetected by quantitative analysis. but conjugated and precipitated with bilirubin, andserved as a network of the pigment stones. These PAS stain positive substances have been identified as glycoprotein and polysac-charid by polyacrylamide gel electrophoresis. Most of these substances could be transformedinto water soluble by soaking into the disodium ethylenediaminetetraacetate solution. Thisis the experimental basis of dissolving pigment stones through "T" tube perfusion.更多还原