【摘要】 <正> 1959年,诺贝尔生理学—医学奖金获得者——Yalow,R.S首先把同位素示踪技术与免疫反应结合起来,建立了胰岛素的放射免疫测定(RIA),使免疫学测定技术的灵敏度整整提高了一个数量级,进入了毫微克到微微克水平。接着竞争性蛋白质结合分析,放射受体分析等多种竞争性放射分析技术相继出现。1967年Catt等建立了涂管固相更多还原

【Abstract】 1 . Solid-phase radioimmunoassay is a newly developed ultra-micro-analytical technique. It posseses the advantage of being very sensitive, highly specific, easy to do and rapid to carry out for the determination. We have established the solid-phase radioimmunoassay technique for alpha-fetoglobulin as a method for detecting primary hepatic carcinoma.2. The principles, experimental conditions and methods for detecting are presented. From the technical point of view, a detail discussion for the anti-bodies to paint the plate and a brief comparison of multiple testing methods for α-feto-globulin are given.3. The results of α -feto-globulin estimation from sera of 51 cases of primary hepatic carcinnoma, 36 cases of hepatis and 24 cases of normalcontrol by means of the antibody to paint the plate method are presented. It is found that the values for all cases of the primary hepatic cancer are above 500 ng/ml and the values for all the hepatitis patients and normal control are below 500ng/ml. Follow-up study are carrying on for the 5 cas es of the latter group with mild elevation of the determination values.4 . The preliminary observations on the method of detecting revealed that the antibody absorption cure and standard curve were stable with rather satisfactory precision and parallelism. This method is capable to obtain a sensitivity of 1.5ng/ml and it takes only 2-4 hours for the completion of the assay.更多还原