【摘要】 <正> T₄RNA连接酶首先由Silber等人在T₄噬菌体感染的E.Coli中发现和初步提纯的。当ATP存在时,它能够催化一定链长的单链寡聚核苷酸5′一端磷酸单酯与3′一端羟基之间形成分子内或分子间的3′,5′磷酸二酯键。RNA连接酶能催化3′,5′二磷酸核苷与寡核苷酸受体相连接。此反应可用于一定序列寡核苷酸的合成,和核苷酸3′一端的同位更多还原

【Abstract】 RNA ligase was first purified from E. coli infected with T4 bacteriophage by the procedure described by Higgins et al. and then subjected to further purification bv an affinity chromatography on blue-dextran sepharose. The enzyme so obtained was highly purified in good yields.It showed one major band on SDS-polyacrylamide electrophoresis. The enzyme was incubated with CpGpGpA and 32pCp under conditions for oligonucleotide joining; homochromatography of the reaction mixture proved that the pentanucleoside penta-phosphate CpGpGpA 32pCp was obtained without any degradation products. Therefore the enzyme purified by this method was found to be free from any detectable ribo-nuclease activity and suitable for the synthesis of oligonucleotides with defined se-quences.更多还原