【摘要】 本文报告了四株(L18株、丹3株、7005株、7009株)钩端螺旋体弱毒株的毒力和免疫原性。实验结果证明,四株弱毒株均不造成地鼠发病和肾带菌,且有较好的免疫原性。其中L18株在地鼠体内只活存三天,不能经地鼠传代,通过地鼠—培基—地鼠连续传10代,未见其毒力增强。用L18株一次免疫(皮下、腹腔内、或肌内接种)地鼠有较好的免疫力,其保护力至少持续九个月(最长观察时间)。更多还原

【Abstract】 The purpose of the present study was to select avirulent strains of leptospira pomona for preparative of live vaccine and to test their safety and efficacy in hamsters and pigs.Four strains ( L18. Dan-3. 7005. 7009) selected were averulent. They were able to make hamsters neither die nor become carrier state and were proved to be highly immunogenic. Amony them strain L18 was studied in detail.1.It was found that leptospires of strain Ll8 might spread to all organs of hamsters after they were inocucated and would clean from the body in three days.The germs could not be transfered by successive passages through hamsters, but they could be transfered by alternative passages through hamsters and in vitro culture and no chang in virulence occurred after such passages for 10 times.2.An effective immunity in hamsters was produced after inoculation of the strain L18 live vaccine. It lasts at least nine months ( observed period) .3.Leptospires of strain L18 were unable to swine become renal carrier state either.70 pigs were alloted into two groups at random. 36 pigs were vaccinated with live leptospires of strain L18 and 34 pigs were not vaccinated as control. After challenge 33 pigs of the control group shed leptospires frequently in their urine, but only 2 of the vaccinated group shed leptospires occasionally.更多还原