【摘要】 本研究工作的目的是从叶绿体老化过程中光化学活性的变化来探讨光合电子传递和膜结构的变化。1.在叶绿体老化过程中,K₃Fe（CN）₆光还原活性的丧失远大于 DCPIP 光还原活性的丧失。2.K₃Fe（CN）₆光还原活性显著下降开始于叶绿体老化12小时之后,而 DCPIP 光还原活性显著下降是在24小时以后。3.DPC 提高老化叶绿体 DCPIP 光还原活性。4.老化叶绿体的光系统Ⅱ氧化侧的破坏大于并早于另一侧（从光系统Ⅱ作用中心至光系统Ⅰ还原侧）的破坏。5.随着叶绿体老化时间的延长,叶绿素含量逐步减少,但下降速度远低于光化学活性下降速度。更多还原

【Abstract】 Chloroplasts were isolated from Spinacia olerecea L.and Doblichos labIab L. Chloroplasts suspension was stored in refrigerator at 5—8℃.Photochemical activi- ties and chlorophyll content of chloroplasts at different times of storage were deter- mined.The results can be summarized as follows: 1.In the course of chloroplasts ageing,the lost of K₃Fe（CN）₆ photoreduction activity was more than that of DCPIP photoreduction activity. 2.The activity of K₃Fe（CN）₆ photoreduction during storage began to decrease markedly after 12 hours,but activity of DCPIP photoreduction began to decrease markedly after 24 hours. 3.The DCPIP photoreduction activity of aged chloroplasts was stimulated by the addition of 1.5-diphenyicarbazide. 4.Destruction of oxidized side of PSⅡ was earlier and higher than that of the other side（from the active center of PSⅡto the reduced side of PSI）. 5.During chloroplasts ageing,the decrease of chlorophyll content was less than the rate of decrease of photochemical activities.更多还原