【摘要】 将大豆(Glycine max(L.)Merr.)哈密瓜(Cucumus melo L.var saccharinus)烟草(Nicotianatabacum L.)和毛白杨(Populus tometasa Carr.)的愈伤组织分别接种在附加IAA4毫克/升和激动素0.5毫克/升的Miller液体培养基上。烟草和哈密瓜的愈伤组织迅速地生长,能持续到50天左右;而大豆和毛白杨的愈伤组织生长缓慢,且20天就停止生长。 测定培养中IAA的消耗情况表明,接种大豆和哈密瓜的愈伤组织的培养液IAA消耗最快,到第四天就消耗完了;接种毛白杨愈伤组织的培养液IAA消耗次之,到第六天也消耗完了;接种烟草愈伤组织的培养液IAA消耗最慢,到第20天仍有加入量的2.8％。 培养液中IAA的消耗与吲哚乙酸氧化酶的活性有密切的关系。吲哚乙酸氧化酶的活性越高,IAA消耗越快,大豆、烟草和毛白杨愈伤组织的生长也随IAA的降低而减慢,但是哈密瓜愈伤组织的生长在IAA消耗完以后,仍能持续生长多日。因此可以认为有些愈伤组织的生长为其所包含的吲哚乙酸氧化酶的活性所制约;有的并不如此。更多还原

【Abstract】 Soybean, aspen, tobacco and Hami melon calluses were incubated each ina 50 ml Erlenmeyer flask which contained 20 ml Miller’s liquid medium sup-plemented with IAA 4 mg/l and kinetin 0.5 mg/l. The tobacco and Hamimelon calli grew more rapidly than the others and their growth continued for aperiod of about 50 days. But the soybean and aspen calli ceased to grow after20 days. The activity of IAA- oxidase in soybean and Hami melon callus arethe highest among the four calli. The next is the aspen callus and the tobaccocallus is the last. The IAA in the media in which soybean and Hami melon calli were incuba-ted was destroyed rapidly and was undetecable afer about 4 or 6 days, while inthe medium with tobacco callus, the destruction of IAA was slow. The IAA inthe medium with callus might be degraded by the enzyme on the surface of or se-creted from the calli. The rate of destruction was related to IAA-oxidase activity.It appears that the growth of the soybean, tobacco and aspen calli is related tothe enzyme activity. The higher the activity, the lower the growth. However,the growth of Hami melon callus is not so.更多还原