【摘要】 为了排除柑桔多胚性对杂交育种的干扰,提高育种效率,进行了成熟种子胚的分离培养研究。证明:一、二级大胚采用普通砂培法,成苗率可达90%以上;在含有GA₃（1ppm）的MS培养基上,三、四级小胚成苗率为80%以上,五、六级小胚也有52.1%发育成苗,为提高杂种实生苗的获得率提供了有效途径。 进一步探讨了各种培养基添加物的生理效应,提出以IAA（1ppm）+CH（400ppm）替代GA₃（1ppm）具有更好的效果,培养的小苗生育健壮,根系发达,为入土成活打下了良好的基础。更多还原

【Abstract】 In order to overcome the interference of polyembryony in cross breeding, and to increase the efficiency in citrus breeding this paper chiefly deals with the technique of separated embryos culture of mature seed.The results indicated that, 1st and 2nd classes large embryos under sand culture, more than 90% of them de-veloped into seedlings. More than 80% of the 3rd and 4th classes small embryos which were cultured on MS medium containing GA3 (Ippm) developed into seed-lings. 52.1% of the 5th and 6th classes small embryos which were cultured on the same medium described above, developed into seedlings too. Thus, the separated embryo culture provides an effective means for increasing the percentage of citrus hybrids plants.The physiological effects of various growth regulators as additives in the me -dium were studied. The results point out that, IAA (Ippm) +CH (400ppm) instead of GA3,was more effetive.The seedlings,which were cultured on this medium,were stronger, and their roots developed better 。 It establishes a solid basis for trans-planting the seedlings into the soil.更多还原