【摘要】 <正> 黄连是毛茛科植物黄连(Coptis chinensis Franch)的乾燥根茎,收载於中国药典为中药中应用较广的一种。近年来我国学者们纷纷报告黄连和其主要成分具有强大的抗菌效用.益增黄连在现代药学上的地他。黄连中含有多种生物鹼以小蘗鹼(berberine)含量最高亦极重要.是黄连中抗菌作用的主要成分。由於小蘗鹼分了结构的特殊(能为三种互变民性体成立平衡状态而存在),所以它的物理性质和化学性质与一般生物鹼不同。这样虽使自黄连中提取小药鹼感觉方更多还原

【Abstract】 (1) A method of isolating berberine hydrochloide present in the Coptis(rhizome of Coptis chinensis) with the aid of chromatography is described. In this method, activated alumina was used as adsorbent and 95% alcohol as both solvent and eluent. The average yield was found to be about 9% of the crude drug. (2) The identification of isolated berberine was accomplished by means of modified circular paper partition chromatography. The R_f value of berberine in the solvent mixture of n-butanol and 3N acetic acid(2:1) was found to be 0.84. (3) A new technique of circular adsorption chromatogram is described. By this method, the content of the berberine in the Coptis has been determined with satisfactory results. The method is simple, readily performed with a minimum of apparatus. The average content of berberine calculated as berberine sulfate in the Coptis was found to be 10.54%.更多还原