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大麦和小麦杂种胚的离体培养及杂种幼苗的形态和染色体观察

EMBRYONIC CULTURE IN VITRO AND OBSERVATION ON THE SEEDLING MORPHOLOGY AND THE CHROMO-SOMES OF THE HYBRIDS OF BARLEY×WHEAT

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【作者】 周之杭; 杜荣骞; 安祝平; 俞新大; 蒋志春; 胡秀英;

【Author】 Zhou Zhihang Du Rongqian An ZhupingYu Xinda Jiang Zhichun Hu Xiuying (Department of Biology, Nankai University, Tianjin)

【机构】 南开大学生物系遗传教研室; 南开大学生物系遗传教研室 天津; 天津; 天津;

【摘要】 用矮六稜裸大麦天津1号与多种小麦品种进行正、反交。授粉后在柱头上滴赤霉酸,刺激幼胚生长,并结合离体培养技术培养杂种幼胚。在几个组合里获得大麦与小麦属间杂种。对杂种幼苗的形态和根尖细胞染色体进行了观察,并且报告了调整培养基中激动素浓度后的效果。

【Abstract】 The intergeneric hybrid between barley and wheat has been obtained by the aid of culture of the young embryo in vitro 9-12 days. At first, only the roots differentiate from the hybrid young embryo and only a trace of the shoot appear. After adjusting the content of kinetin in the medium, the normal differentiation of the shoot tips is observed. There are 28 chromosomes for Tianjin 1 barley×Xiaoyan 58 wheat most cells being in the root tip of the hybrid. This number fits in well with the expectant (7 + 21). But cells containing 21 or 49 (7 + 42) chromosomes are also found. The latter contain 7 haploid chromosomes of barley and 42 diploid of wheat.

  • 【文献出处】 遗传学报 ,Acta Genetica Sinica , 编辑部邮箱 ,1979年03期
  • 【被引频次】26
  • 【下载频次】52
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