【摘要】 由6个苹果品种和1个梨品种的幼胚下胚轴及子叶基部直接诱导出不定芽。不定芽的发生是成从的。实验使用MS作基础培养基,其中附加细胞分裂素和生长素的浓度为BAP 1—3ppm,NAA 0.5ppm。但是,我们在进一步实验中表明,在上述培养基中除去了生长素亦得到同样结果。在含有0.5ppm IBA的土豆培养基上,离体诱导下胚轴及子叶基部处在发育中的不定芽生根,成长为完整植株。这一结果为克服果树及农作物杂交育种中常见的幼胚不育和扩大繁殖优良品种的无性系植株,提供一个可能的实用方法。更多还原

【Abstract】 Adventitious buds derived from the hypocotyl and cotyledon in the early and young embryos in six strain’s of apple and in one strain of pear have been successfully induced. These buds developed in a cluster. In our experiment MS medium was used as the basic medium and was supplemented with 1-3 ppm BAP and 0-5 ppm NAA. But in our further experiments similar results were obtained with the above mentioned medium devoid of the growth substance. In the medium of potato juice with the addition of 0.5 ppm IBA the in vitro induced shoots derived from the hypocotyl and from the base of the cotyledon of the young embryos formed roots and eventually developed into the whole plantlets. This result may provide a practical method for overcoming the sterility at the young embryo stage as commonly occured in cross breeding of the pomological and the cereal plants and for the asexual multiplication of the clones of these cultivated plants.更多还原