【摘要】 研究了水稻（Oryza sativa）花药的离体培养及其花粉植株后代的表现,得到如下结果: 1.用加有2,4-D 1—5毫克/升的Blaydes培养基培养水稻花药,诱导出水稻花粉愈伤组织。采用花粉处于单核期的花药进行培养比较合适。 2.诱导愈伤组织成苗,以二次诱导法效果较好。即将愈伤组织种植在低浓度激素的培养基上（IAA 0.05—0.5毫克/升,激动素1—2毫克/升）,在此培养基上分化快,芽点多,但芽不易伸长;芽点出现后再移到激素浓度较高的培养基上（IAA 2毫克/升,激动素4毫克/升）,很快出现幼苗。 3.水稻花粉植株二代,田间表现和室内分析结果表明基本整齐一致,没有分离。杂种F₁花粉植株后代在许多性状上超过双亲,有选种价值。说明水稻花药培养用于育种是可能的。更多还原

【Abstract】 Having been studying the anther culture in vitro of rice (Oryza sativa L.) and the expression of the progenies of pollen plants, the results we have acquired are briefly summarized as follows:(1) In order to induce the callus from rice pollen, the medium recommended by Blaydes is used as a basic medium with addition of 1-5mg/l 2,4-D. Anther contained uninucleate pollens are found to be the optimal stage for culture.(2) The double induction methed is effective to induce the differentiation of shoots pollen callus. Namely, in the first stage, the pollen callus are planted in the medium which contains low concentration of IAA (0.05-0.5mg/l) and Kinetin (1-2mg/l). In this medium, the callus differentiates quickly and a great number of bud spring up, but they grow slowly. In the second stage, buds after their appearance, are transferred to the medium which contains high concentration of IAA (2mg/l) and kinetin (4mg/l). And then, the callus soon gives rise to shoots.(3) The results of examination both in the fields and in the laboratories show that the secondary generation of the rice pollen plants are uniform and have not any differentiations. Many characters of the progenies from the hybrid F, of the rice pollen plants are better than their parents. These characters are of value to breeding. It can be believed that the anther culture of rice is a hopeful and feasible breeding methed.更多还原