【摘要】 <正> 1888年Kunstler氏首先在法国看到牛胎毛滴虫(Trichomonas foetus),当时他指出,“牛的阴道中寄生有毛滴鞭毛虫,同样地在猪的肠道中也寄生有其他的鞭毛滴虫。”后于1900年Mazzanti于意大利从二个母牛和一个公牛的生殖道中发现了牛胎毛滴虫。牛胎毛滴虫虽在1900年以前早已发现,但是直到1924年病理学家们对于乳牛流产病原问题都把注意力集中在流产蒲氏杆菌Brucella abortus上,而Hopfengartner1925年在Dreseher的发现就被忽视了。终于1928年Riedmuller的经典著作肯定更多还原

【Abstract】 In the course of our studies on human trichomoniasis, efforts have been made to elucidate some morphological and physiological differences between human and bovine trichomonas. Aborted foetus, uterine pus, amniotic and allantoic fluid of infected cows were obtained from dairy farms in Shanghai for isolation of T. foetus. Bacterial-free cultures of several strains of T. foetus were successfully established in our laboratory, and the organisms have been maintained in vitro over a period of 10 months for various experiments. The present paper is a preliminary report on methods of bacterial-free cultures of T. foetus and culture-characteristics of the bovine trichomonas in various culture media. 1. Methods for isolation and cultivation of T. foetus are herewith described in detail. 2. Different culture media: F-bouillon, V-bouillon, Noguchi and Ohira’s medium, Nutrient agar slants were tried, and it was found that F-bouillon yielded the best results. 3. Method for cultivating T. foetus in solid medium is described. T. foetus multiplied and different sizes of round colonies formed on the surface of plated medium. 4. A brief review of the bacterial free culture of T. foetus is presented in the discussion and the pathogenicity of T. foetus in comparison with that of T. vaginclis was taken into consideration from the viewpoint of the host and parasite relationship. Bovine trichomoniasis is quite a common veterinary disease in our country and yet little attention has been given to this problem, consequently knowledge regarding its epizoology and preventive measures adaptable to the rural conditions of this country has been very vague. The surging tide of socialism in China’s countryside has swept the country with prosperity and bumper harvests. Naturally prevention and treatment of bovine trichomoniasis will soon become one of the urgent demands from our agricultural producer co-operatives, dairy farms and national collective farms. In order to keep pace with the speedy development of socialist transformation, the present paper points out the importance of an immediate intensive study of bovine trichomonas.更多还原