【摘要】 初步研究了 C₉₀号菌在摇瓶发酵中产生反丁烯二酸（以下简称反丁）的条件。该菌在6%液体石蜡（以下简称液蜡）和5%试剂级碳酸钙等条件下发酵74—85小时产反丁可达3.8—4.2%,转化率为63—70%,在流加试剂碳酸钙情况下,发酵162小时可达5.15%,转化率在85%左右。在用工业碳酸钙等情况下,发酵72小时,产反丁2.5%以上。不同碳、氮源对产反丁影响很大。最适碳源是 C_14-18液蜡。最适氮源是尿素,其次是硫酸铵和氯化铵。培养基中必须加入磷酸二氢钾、酵母汁和碳酸钙。锌离子对产反丁不利。在普通500毫升三角瓶中,装液量对产反丁影响很大,但在挡板瓶中,装量30—90毫升,对产反丁影响不大。此外摇床速度、种龄、接种量对产反丁亦有很大影响。更多还原

【Abstract】 Fermentation conditions for the shake flask production of fumaric acid by Candida rugosa strain C₉₀ were inve- stigated.After 74—85 hr.of fermenta- tion in a medium containing 6% n-pa- raffin and 5% reagent grade CaCOs, the yields of fumaric acid were 3.8— 4.2% （conversion rate 63—70%）.It was further increased to 5.15% （conver- sion rate 85%） by continuous feeding of CaCO₃,but when using industrial grade CaCO₃,the yield was only 2.5%. The suitable chain length of n-paraffin is C_14-18.The suitable nitrogen sources are urea and （NH₄）₂SO₄ and NH₄Cl. Phosphate and yeast extract are nessarry for acid production,zinc ion is inhibi- tory.Besides that,the rate of aeration and the age and amount of inoculum also greatly’ effect on acid production.更多还原