【摘要】 本文报告了用含有10%牛血清的#~199综合培养基,培养了经过胰蛋白酶分散的鼠胚肺组织细胞。用普氏立克次氏体鼠肺毒株感染了单层细胞,不加抗菌素在34℃培养,立克次氏体的繁殖开始于48—96小时,高峰在4—7天,约有80%的细胞受到感染。立克次氏体的发育过程是:丝状体→链状体→长杆状→短杆状。有时在丝状体的前一阶段时间内先有始体出现。在油浸接物镜下看到的细胞病变是:1)梭形及多边形上皮细胞被极端增殖的立克次氏体所胀破;2)这两种上皮细胞的退化和碎解;3)游离到细胞外的立克次氏体。至于星状上皮细胞内立克次氏体的增殖较其他类型的上皮细胞中的增殖为早。巢形细胞在感染的早期出现空泡性变化,后期细胞脱落,立克次氏体寄生于空泡之间的间隙中。组织培养收获物(细胞)经鼻腔感染小白鼠证明有致病力,且可在鼠肺涂片上找到大量的立克次氏体。据估计,普氏立克次氏体在鼠胚肺组织培养中的增殖量至少为原接种量的十几倍。因此,可以考虑用本法制备立克次氏体抗原和进行疫苗的研究。更多还原

【Abstract】 1. Trypsinized mouse-cmbryo-lung tissues werecultivated in the #199 synthetic medium containing10% calf serum and antibiotics.Monolnyers thusformed were infected with a suspension rich inRickettsia prowazeki without antibiotics.Incuba-tion was carried out at 34℃.Visible rickcttsialmultiplication began at 48 to 96 hours and thepeak of multiplication was 4 to 7 days after in-fection.About 80% of the cells were found to beinvaded by the rickettsia.The developmental cycleof intracytoplasmic rickcttsiae was as follows:thread-like forms,strepto-bacillary forms,bacillaryforms,and cocco-bacillary forms. It is proposed to employ the term"initial bo-dies"instead of homogeneous inclusions,as it isassumed that the initial bodies occur only duringthe adaptation of the rickcttsial strain to a newtissue-culture environment. 2. Forms of epithelial cells closely similar tothat described by Zaslavsky for the monkey renalcells that have been seen both in the infected anduninfected monolayers.Cytopathogenic effects(CPE)could be visualized only with the aid of an oilimmersion objective.These were:(1) bursting ofthe spindle-shaped and polygonal epithelial cellsdue to overcrowding of proliferating rickcttsiae.(2)cellular degeneration and disintegration and (3)extracellular migration of rickcttsiae.The appea-rance of multiplying rickettsiae was first in thestar-shaped epithelial cells.There was markedvacuolization in the nidular cells of the epithelialtype in the early stage of infection but it decreasedlater,when luxuriant intracytoplamic parasitizedrickcttsiae could be seen in between the vncuoles. 3. Fibroblasts appeared to be hardy cells.Even though thev were over-laden with rickettsiaeand were stained much deeper than usual,yet theirReneral morphology was well preserved. 4. Intranasal instillation of the harvested tissueculture material to mice was shown to be patho-genic.Rickettsiae were abundant in lung smearsof the infected mice. 5. It is estimated that the multiplication ofR. prowazeki in the monolayer cells of mouse-embryo-lung tissues is at least ten times more thanthat contained in the original inoculum.更多还原