【摘要】 本实验共用雄性猕猴(Macaca mula-ila)12只,分成三组:(1)对照组;(2)X射线200仑一次急性照射后第9天取出一侧睾丸,作为一次急性照射组;(3)把200仑总剂量分成10份,每隔24小时照射20仑,最后一次照射后的第9天取出一侧睾丸,作为分次累积照射组。一次照射组和分次照射组中A型精原细胞和B型精原细胞数量都明显低于对照组。一次照射情况下,该两类精原细胞各占对照的65.43%和33.09%;分次照射时各占对照的5.57%和0%;畸变细胞百分率在分次照射组中要比一次照射组高2.3倍。一次照射和分次照射后精母细胞数量都下降,分别占对照组的84.27%和6.92%,但只有分次照射组与对照组的差异是显著的(P<0.001)。畸变精母细胞在分次照射组中高达50.21%,并分布在67.5%的精细管切面内;一次照射后,畸变精母细胞较少,只有0.78%。随着精细管切面直径的收缩,支持细胞数量有所增加,尤以分次照射组为甚,在统计学上有显著意义。上述结果表明,分次累积照射对生殖细胞的损伤效应比一次急性照射严重,可能是射线对生殖细胞直接致损和抑制细胞分裂两者综合作用的结果,至于两类精原细胞的受损程度和数量消减的程度都不一致,可能是辐射敏感性差异的反映。更多还原

【Abstract】 This paper presents the results ofthe observations on the quantitativechanges of type A and type B sperma-togonia, spermatocytes and Sertoli cellsin the testes of the monkey, Macacamulatta, following single and fractionateddosage of exposure to X-irradiation. Thematerials consisted of 12 testes, whichwere obtained from 12 different monkeysand were set into three different groups:(1) the control, (2) single dosage ofexposure to 200 r X-irradiation and (3)fractionated dosage of exposure to 200r for 10 consecutive days with 20 r each. The results show clearly that thereexists a marked difference in both typeA and type B spermatogonia in thefrequencies between the control and theirradiated animals, and that the meanfrequency of these cells is significantlyhigher in the single exposure group thanin the fractionated group. In both cases,the frequency of spermatocytes is lowerthan in the control, though the diffe-rence between the single exposure groupand the control is not statistically signi-ficant (P>0.05). In both radiated groups,the frequency of Sertoli cells rises asthe diameter of the cross section of theseminiferous tubules, on the contrary,diminishes gradually. The percentagesof aberrant cells are greater in theradiated groups than in the control. Among the different types of cells,there exists an apparent heterogeneity inradiosensitivity. The results further sug-gest that the greater damage caused bythe fractionated dosage of exposure thanthe single dosage of exposure may beascribed to the integrated effects ofradiation, which acts through the directkilling of spermatogonia on one handand the inhibition of mitotic divisionson the other.更多还原