【摘要】 用兔抗羊 LH 血清和放射性碘标记的羊 LH 建立了大白鼠血清中 LH 的双抗体放射免疫测定法。在这异源的系统中羊 LH 和大鼠 LH 的剂量反应曲线是平行的。当用羊LH_2-2-1作参考标准时测量的灵敏度为0.1ng/管。当用大鼠 LH-RP-1作参考标准时测量的灵敏度为10ng/管。大鼠的 FSH 没有交叉反应,所以 FSH 不会干扰血清 LH 的测定。用这个系统我们研究了去卵巢以及去卵巢后给予孕酮和巳烯雌酚以后大鼠血清中LH 水平的变化。结果表明性类固醇激素对大鼠垂体 LH 的分泌具有一个负反馈的调节机制。本文中还研究了棉酚对大鼠 LH 分泌的影响。我们发现成年雄鼠服用棉酚以后血浆 LH 的水平以及垂体对 LRH 的反应均无显著的变化。结果表明新的男性抗生育药物——棉酚似乎不影响垂体的 LH 分泌功能。更多还原

【Abstract】 A double antibody radioimmunoassay for detecting luteinizing hormone inrat serum was developed by using rabbit anti-ovine LH serum and radioiodinatedovine LH.In this heterogeneous system the dose-response curve of ovine LHwas parallel to that of rat LH.When ovine LH_2-2-1was used as a referencestandard,the sensitivity of the assay was found to be 0.1 ng per tube.The im-munoassay potency of LH_2-2-1was equal to that of NIH-LH-S₁₈.By using ratLH-RP-1 as a reference standard,the sensitivity was found to be 10 ng per tube.Since rat FSH did not show crossreaction with anti-ovirie LH serum,the inter-ference of FSH appeared to be negligible in the measurement of serum LH.By using this assay system we have studied the changes of serum LH levels inrats after ovariectomy and with treatment of progesterone and diethylstilboestroltwo months after the operation.The results confirm that sex steroid hormonespossess a negative feedback mechanism to pituitary LH secretion.The effect ofgossypol on LH secretion has also been investigated.It has been found that inadult male rats there are no significant changes either on the serum LH level or inthe response of pituitary to LRH injection following gossypol administration.The data seem to indicate that this new antifertile agent for male,gossypol,does not influence the LH secretion of pituitary.更多还原