【摘要】 <正> 细菌性痢疾为常见的肠道传染病,发病率较高,于集体生活的人群中更容易传播和流行,严重影响人们的健康和劳动力。常规的细菌培养较费时、阳性率也不高,因此,如何早期快速诊断细菌性痢疾,寻找灵敏、可靠的快速检测方法,对于及时确诊和积极防治菌痢均具有重要意义。本文对采用免疫荧光菌球法检测痢疾患者粪便标本的资料作一总结,并就免疫荧光菌球法及其在诊断细菌性痢疾的作用进行探讨和评价。更多还原

【Abstract】 The principle of IFBC technique was to grow stool specimen in presence of Fluovesoence-labelled (FL)-antidysentery (F2a, F3, Sonne) sera and imported Tryp-tose, with or without the addition of chloromycetin 12μg/ml, on slide at 37℃ for 8-12 hours. At the end of incubation period, a single dysentery bacillus would have grown into a colony, which could be viewed as a flourescent ball under flourescent microscope. Using this method, 109/140 (77.85%) specimens from 134 cases of acute baoillary . dysentery received during July - November, 1978 were positive, while the positive rate by conventional cultural method performed simultaneously was only 41/140 or 29.28%. Thus, the IFBc technique was much more sensitive and time saving than the routine cultural method. However, there were 4 specimens positive by cultural but negative by IFBc technique. The inooporation of ohloromycetin into the FITC-antidycentery sera seemed to suppress the growth of nonpathogenic, sensitive microorganisms, thus facilitating the recognition of colony of dysentery bacilli. In order to isolate pure culture for further identification and for tests for sensitivity to antibiotics, slide culture was flashed with sterile saline solution through filter paper set in a small filter, and the paper filter disc was then removed and cultured on S. S. agar overnight. As a result, 4/109 specimens, positive by IFBc technique failed to grow on S. S. agar. These negative results were likely due to faulty technique and are being investigated. In spite of these ,we feel this new method was a very sensitive and rapid one as compared with the routine cultural procedure, and was moreover more sensitive and reliable than direct staining of stool specimens with FL-antidy-sentery sera without incubation in antisera overnight. Further, owing to its simplicity, it could be carried out in field work where cultural facilities are not available.更多还原