【摘要】 1.赤血球凝集反应用纯卵冷浸抗原有一定的灵敏性和特异性。本实验239例血吸虫病患者,阳性反应者有222例,阳性率为92.9%,一般血清稀释效价变动在1:40～1:320之间,可高达1:2,560,而急性血吸虫病例曾高达1:20,480,对其他寄生虫病尚未发现交叉反应。更多还原

【Abstract】 In the performance of the test,the original technic of Boyden (1951) has been followed with some modifications. Sheep cells can be preserved in the mproved Alsever’s solution of Kendrick in refrigera- tor for 3 months without evident hemolysis. The test antigen,1:400 in ph 6.4 buffered saline,to be used for the sensitization of the tanned sheep cells, is stable for 2 months at -10℃.Reactions are recorded as “-”,“+”,“++”,“+++”,in different serum dilutions after the critetion set up by Stavitsky with a slight-modification. 1:20 serum dilution has been considered as the minimal effective titre. The time for the occurrence of positive haemag- glutination reactions in rabbits after innoculation was in proportion to the degree of infection,averaged 17.3 days in the group with heavier infection (2000 cercariae each) and 23.7 days in the one with lighter infection (100 cercariae each). On the ayerage,stool hatching became positive in 37.3 days following infection for the former and in 42,6 days for the latter. Therefore,this reaction hasita value of early diagnosis in experimental schistosoma infection. A single haemagglutination test in each of the 239 cases of schistosomiasis revealed a positive rate of 92.9%. The effective titers for serum dilution most commonly encountered in chronic cases were found to be laying between 1:40-1:320,with a highest value of 1:2560,while in acute cases.1:640-1:20,480.All the 28 mormal persons and 24 non-schistosoma medical cases showed negative reactions.In one case of subacute bacterial endocarditis with neither de- finite history of exposure nor any clinical evidence of schistosoma infection,yet the reaction was positive (similar with other serological tests as cercariae membrane,circumova precipitin and carmine floccu- lation reactions).Whether the bacterium in question (not isolated) shares common antigenic fractions with schistosoma worms should call for further attention. No cross reactions in cases of leishmaniasis. amoebiasis,giardiasis,paragonimiasis,clonorchiasis. ancylostomiasis,filariasis,ascariasis and taeniasis have so far been encountered. In conclusion,it has been pointed out that the chief advantage of thins test lies in the high serum- dilution titre,which far surpasses other serological methods used in this infection as complement fixation test,etc. Thus,if not only gives us a means to titrate the antibody content of the serum under examination but also enables us to follow up the course of the disease.更多还原