【摘要】 按前报用分子筛凝胶柱层析纯化家蚕细胞质多角体病毒(Cytoplasmic polyhedrosis virus,以下简称CPV)作为抗原制备了兔抗血清。对接种CPV后不同时间的家蚕中肠组织内CPV的增殖过程进行了观察,表明利用免疫对流电泳在CPV感染后的12～20小时(即病毒在病蚕中肠组织内增殖初期)就可检出。家蚕CPV的抗血清与其他几种昆虫的CPV有免疫交叉反应,但当家蚕CPV的抗血清经双链多聚核苷酸(肌苷酸/胞嘧啶核苷酸,即poly I/poly C)吸收后,则与其他几种昆虫CPV的交叉反应即行消失。更多还原

【Abstract】 The multiplication of the cytoplasmic polyhedrosis virus in infected midguts of silkworm can be detected 12～20 hr after inoculation by means of counterimmunoelectrophoresis. This technique may prove to be of value in the practice of sericulture.The antiserum of cytoplasmic polyhedrosis virus of silkworm and of cytoplasmic polyhedrosis viruses of other insects such as Theophila mandarina Moore, Rondatia menciana Moore, Arctornis Chrysorrhoea Linne and Heliothis armigera Hubner showed cross-reaction. But if the antiserum of cytoplasmio polyhedrosis virus of silkworm ha previously been absorbed with poly (I):(C), these cross-reactions disappeared.更多还原