【摘要】 经不同温度（从30°至58℃）处理的离体蛙视网膜,冷却后在室温（15℃左右）的情况下引导ERP,R₂振幅随处理温度不同而有不同的变化,处理温度不超过40℃,对R₂几乎没有影响,其振幅与对照组相近。用40℃以上至50℃以下的温度处理后,R₂显著加大,尤其是47～48℃处理后,R₂达最大值,为对照组的2.57倍。用50℃及更高温度处理后,R₂又急剧下降,且低于对照值。经58℃处理后,R₂基本消失。此外,这种变化还与处理时间长短有关,若缩短加温时间,则分别需要在更高一些的温度处理,R₂振幅才依次增大、减小和消失。本文对上述变化,从温度影响视细胞外段膜结构液晶态的流动性和有序性的角度,进行了初步的讨论。更多还原

【Abstract】 Isolated frog retinas were kept for 10 minutes at various temperatures, then cooled to the ambient temperature （about 15℃） and ERP was elicited with flash stimulation. The results showed that R₂ amplitudes underwent different changes with retinas treated at different temperatures. It was not until above 40°C that the R₂ amplitude began to show enhancement. R₂ increased when the temperature was raised to above 40℃ and remained so till the temperature rose above 50℃. The augmentation of R₂ amplitudes was particulary marked at 47～48℃, the R₂ amplitude rising to 2.57 times that for retinas without treatment. But when the temperature was raised above 50℃, the R₂ amplitude decreased and went below the average for the control group. At 58℃, R₂ was almost abolished.In addition, these changes of R₂ amplitude also depended on the duration of heating, the shorter the duration of heating, the higher the temperature necessary for the same changes of R₂ amplitude. Therefore, with shorter heating times the R₂ amplitude was enhanced, decreased and abolished respectively at relative higher temperatures. The relationship between changes observed in R₂ amplitude of ERP and changes of order and fluidity of the liquid （?）rystals of the lamellar membranes of the visual cell was also briefly discussed.更多还原