【摘要】 对于食物中N-亚硝胺类致癌物质的含量虽可用气相层析——质谱仪方法进行测定,但由于仪器价格昂贵,在一般实验室较难普及。因而,我们研究了食物中挥发性N-亚硝胺总量的定量比色测定。方法包括溶剂抽提、水蒸汽蒸馏、紫外光解释放亚硝酸根进而用离子交换树脂浓缩、洗脱、显色定量。总回收率为70～110%。可测至10^-8克水平。更多还原

【Abstract】 During the past ten years,N-nitrosamines has attracted a great deal of attentionas a carcinogenic hazard in the environment.The monitoring of trace amounts of N-nitrosamines in food is now possible with gas-chromatography mass-spectrometry,if thecostly instruments are available.But for routine analysis in an ordinary laboratory thisis hardly feasible.A simple colorimetric method is proposed here for the detection of the total volatileN-nitrosamines in food extracts.The method involves solvent extraction,fractional dis-tillation and ultra-violet irradiation to liberate nitrite,followed by concentration witha strong basic resin.The nitrite is then eluted with sodium chloride and detected withthe Griess reagent.The recovery is of the order of 70～110per cent at the 10^-8g level.更多还原