【摘要】 (1)本报告提供根据瓜氨酸砷酸解产物的鸟氨酸及氨的生成量作为测定鸟氨酸氨基甲酰移换酶(OCT)活力的两种方法。(2)根据氨生成量的直接纳氏比色法是最快速简捷的方法,重复性及回收率均很高,所需设备亦较简单;根据鸟氨酸生成量的纸上层析法,能测极微量的样品,应用两种方法,均获得相同的结果。(3)对OCT的最适温度、pH及酶、底物浓度等影响作了研究。(4)OCT对热有高度的稳定性。更多还原

【Abstract】 Two methods for the determination of ornithine carbamyl transferase (OCT) activity, by estimating the products of arsenolysis of citrulline, ornithine and ammonia respectively, are described.One method,based on the direct Nessler reaction with ammonia, is a simple, rapid and accurate method, with satisfactory recoveries.The other method, based on the determination of ornithine by means of paper chromatography, is a micromethod and gives results similar to the first method.Optimal conditions with respect to pH, temperature and substrate concentration were investigated. The relatively high temperature optimum was explained as due to the heat stability of the enzyme.更多还原