【摘要】 本实驗初步观察了大黃酸和大黃素对金黄色葡萄球菌核酸与蛋白质的合成,氨氮的同化利用,氨基酸的氧化脫氨和轉氨作用的影响。（1）大黄酸和大黄素对金黃色葡萄球菌在葡萄糖-肉湯培养基中RNA、DNA和蛋白质的合成具有很强的抑制作用。1抑菌单位濃度的大黄酸（15微克/毫升）,其抑制百分率分別为90、84和72;大黄素（10微克/毫升）分別为100、100和93。（2）叶酸对大黄素抑制金黄色葡萄球菌核酸的合成有拮抗作用,大黃素在最低抑菌濃度(3.6×10^-5M),叶酸濃度为5.6×10^-4M时,可使RNA和DNA的合成分別恢复47&和40%。（3）大黄素对金黄色葡萄球菌在含葡萄糖和硫酸銨培养基中氨氮的同化也有抑制作用,在最低抑菌濃度抑制率为92%。（4）大黄素即使高至200微克/毫升的濃度,对金黃色葡萄球菌谷氨酸-丙氨酸轉氨作用的影响不明显。（5）大黄素对金黄色葡萄球菌氨基酸的氧化脫氨具有抑制作用,对谷氨酸和精氨酸的抑制最强,甘氨酸和丙氨酸次之,門冬氨酸和絲氨酸最弱。对耗氧和脫氨的抑制有平行的关系。更多还原

【Abstract】 (1) The biosyntheses of RNA, DNA and protein in S. aureus in ordinary broth containing glucose are strongly inhibited by emodin and rhein of minimal growthinhibitory concentration （10γ/ml and 15γ/ml respectively）. The percentages of inhibition for rhein are 90, 84 and 72 respectively, while those for emodin are 100, 100 and 93 respectively.（2） Folic acid exhibits antagonistic activity to the inhibitory action of emodin on RNA and DNA snytheses. In presence of 3.6×10^-5M emodin and 5.6×10^-4 M folic acid, the percentages of recovery of RNA and DNA syntheses are 47 and 41 respectively.（3） At the minimal growth-inhibitory concentration of emodin, the assimilation of ammonia in S. aureus is inhibited by 92%.（4） Emodin, even at a concentration of 200γ/ml, shows only very slight inhibition on the glutamate-alanine transamination system, as far as can be judged from paper chromatogram.（5） Emodin also inhibits the oxidative deamination of certain amino acids in S. aureus. Among the amino acids studied, glutamate and arginine are affected notably, glycine and alanine are also affected but to a lesser extent, while aspartate and serine are almost unaffected.更多还原