【摘要】 利用相差显微镜,并辅以涂片染色、化学反应方法,将粘虫各虫态虫龄的血细胞分为8个类型。他们是:原血胞、浆血胞、粒血胞、珠血胞、类绛血胞、囊血胞、梭血胞及脂血胞。并统计出各类血细胞在粘虫一生中的数量变化。本文认为足血胞与粒血胞不同之处在于前者的伸延部分含有细胞质。符合这一标准的足血胞在此次工作中看到的不多,因此对粘虫足血胞的存在问题暂作保留。 苏芸金杆菌处理后,粘虫血细胞产生了明显的病态变化,随着病症的进展,非正常血胞自2％上升为30％,50％,83％左右。直到死亡,血淋巴内未发现杆菌出现。更多还原

【Abstract】 Supplementing previous studies of stained smears of the hemocytes of Pseudaletiaseparata the present communication reports on the results of investigations made withthe aid of phase contrast microscope. Eight of the nine types of insect hemocytes, asrecognized by Jones (1962), have been identified in the hemolymph of Pseudaletia se-parata, namely, prohemocyte, plasmatocyte, granular hemocyte, cystocyte, spherule cell,oenocytoid, adipocyte, vermiform cell. The constant occurrence and numerical variationof each of these types in the different stages from the first instar to the adult have beendetermined. A characteristic appearance of the granular hemocytes is the sending off of spinelikeextentions from a semitransparent homogeneous border of the cell-body. Occasionallyhemocytes with extensions distributed radially over the surface may be observed. By con-tinuous observation under the microscope and determination of its reaction to stain intro-duced under the coverslip, it was ascertained that the ultimate form of these two typesshows no difference and that the chemical reaction is identifical. From this it is con-cluded that both these types are granular hemocytes, altho it is easy to confuse the granu-lar hemocyte with radial extentions with the podocyte. It is stressed that the criterionfor identifying a podocyte is the continuation of the cell cytoplasm into the extensions.A small number of hemocytes conforming to this criterion has been observed but wewould like to reserve our opinion on the presence of podocytes in Pseudaletia separatauntil further studies shall have been made. In the course of the present study it was also clearly demonstrated that pathologicalmodifications of the hemocytes—such as vacuolation of the cytoplasm, crenulation or ir-regularity of the cell border, uneven distribution of the cytoplasm, eccentricity of thenucleus, condensation of granules in the nucleus—occur prior to the entry of the patho-gen, Bacillus thuringiensis var. thuringiensis into the blood of the armyworm. While thesignificance of such a phenomenon will be discussed in another paper, it is here pointedout that the occurrence of these modifications in the hemocytes affords a ready means forstudies on the pathology of bacteriosis in Pseudaletia separata, one of the worst pests toagriculture in this country.更多还原