【摘要】 <正> 乳腺发育各阶段的形态学变化,文献上屡有记载;但有关乳腺的组织化学变化,除碱性磷酸酶有较多的报告外,在糖原、核酸、类脂质等方面,仅Dempsey等及Montagna等做过部分的观察。我们希望通过本实验能对小白鼠乳腺各机能时期的核酸、碱性磷酸酶、糖原、类脂质的变化作系统的说明,并探讨上述物质与机能之间的关系,以作为进一步实验研究的依据。更多还原

【Abstract】 The histochemical changes of the resting, pregnant, lactating and involuting mammaryglands of ninety mice (virgins and first pregnancy) aged 2--3 months have been studied.The changes of nucleoproteins, alkaline phosphatase, glycogen and lipid were studied bymethods of methyl green-pyronin, Gomori’s Ca-Co method, periodic acid-Schiff’s reagentand Sudan black stain respectively. The morphological changes were demonstrated byH-E stain. RNA was observed in the cytoplasm and neucleoli of the glandular epithelium. Thecontent of RNA increased gradually from midpregnancy and reached maximum duringlactation. During involution it decreased rapidly, and recovered to the normal level atthe 6th day after weaning. In the glandular epithelium the alkaline phosphatase was more evident in the restingstage. During pregnancy it weakened gradually. It was minimal during lactation andincreased markedly after weaning. The content of alkaline phosphatase was more in theactive-secreting cells than that of the resting cells. Myoepithelial cells always gavestrong reaction. In the glandular epithelium glycogen was only present in a few lactating mammaryglands, and absent in all the others. During late pregnant and lactating stage, the glan-dular epithelium was weakly positive by PAS stain. Lipid was observed in the cytoplasm of the glandular epithelium. The content ofit increased gradually from midpregnancy and reached the maximum during lactation, butdecreased during involution. After weaning, lipid granules were present only in thelumen of the duct. We did not find any significant changes of the mentioned substances during theestrous cycle. The relationships and the functional significances of the mentioned substances werediscussed.更多还原