【摘要】 本文提供了一个以724离子交换树脂为吸附剂,从人尿中分离尿激酶粗品的有效方法。此法是:将1吨新鲜的男性尿调到pH9.O,除去沉淀,滤液调到pH 5.8—6.O,然后在滤液中加入1.5％（W/V）724树脂（H⁺型）,搅拌1.5时,被吸附的尿激酶以2％的NH₄OH（含1M NaCl）溶液洗脱,在洗脱液中加入（NH₄）₂SO₄使达O.6饱和度,收集沉淀,收率为60—70％,比活为1000CTA单位/mg·pr。更多还原

【Abstract】 Crude urokinase separated from human urine was first subjected to chromatography of exclusion on DEAE-eellulose, with pH6.4 and then was purified by chromatography on a column packed with DEAE-cellulose which had been previously equilibrated with the glycine buffer at pH9.2 and 0.6mΩ^-1. and eluted with the above buffer at pH8.8 and 6-7mΩ^-1. Urokinase solution of specific activity～5000 CTA units/mg. protein was passed through a 724 resin column treated with a 0.01M Na₂HPO₄ solution, washed with the same buffer and eluted with a pyrogen-free solution contaning 0.01M Na₂HPO₄ and 0.5M NaCl. The yield was～40% and specific activitywas～40000—50000 CTA units/mg.protein.更多还原