【摘要】 <正> 海水中营养要素硝酸盐的测定方法,长期来一直是化学海洋学中一个较难解决和颇为关注的研究课题。 本文作者(1963)曾制成一新试剂,即N,N′一双(对磺苯基)联苯胺,或二钠盐[N,N′-bis(p-sulphophenyl)benzidine(or disodium salt),简称BSPB],亦即二苯基联苯胺二磺酸钠(此名磺基定位不确切,故以本文命名为妥),并用于比色测定海水中硝酸盐。此法原理为:硝酸盐于硫酸介质中及氯离子存在下,可将BSPB氧化为醌型BSPB紫,进行比色测定。更多还原

【Abstract】 In this paper a simple rapid colorimetric method for determining the nitrate in sea water with BSPB [N, N’-bis (p-sulphophenyl) benzidine disodium salt] is recommended. It was developed by further improving the conditions reported in the previous paper published in 1963 by the author, and was shown to be more stable, sensitive and suitable for the routine analysis of sea water.The procedure of the determination is as follows: to 2 ml of sea water sample add 0.05ml of 0.005 M CuSO4 solution, 5ml of H2SO4 (4:1) and 1 drop of 0.4% BSPB solution, mix, and allow to develop for 25 min. Then add 1 drop of 0.05 M hydrazine sulphate solution and submit to colorimetric determination after thoroughly mixing. The measurable range of the determination is from 1 to 100μg NOs-Nl-1 with an average error 4%. This method has been tested in the oceanographic research of the East China Sea in 1966.Studies were also carried out on the purification of BSPB by recrystallization and some properties of its solution.更多还原