【摘要】 应用浓度梯度培养皿的方法,在Escherichia coli K12 W1177和K12 W1485 his~-系统中测定了93种药物对于溶源性细菌释放噬菌体的诱导能力.这些药物包括已知的基因诱变剂、抗肿瘤药物、致癌药物、辐射防护剂以及代谢抑制剂等.诱导量达到5%(约25倍于自发释放量)的药物共15种,诱导量在45%以上的有3种.在具有诱导作用的药物中至少有60%的药物对于基因有诱变作用.有6种药物对于自发释放有抑制作用,其中尿嘧啶的结构类似物占4种.实验表明许多同时具有致癌和抗肿瘤作用及诱变作用的药物都具有诱导作用.文中简单地讨论了药物的杀菌作用和诱导作用之间的关系,以及5-溴脱氧尿核苷和重氮尿嘧啶及2-硫尿嘧啶等天然碱基结构类似物的可能作用机制.更多还原

【Abstract】 By means of the gradiant technique, 93 different kinds of chemicals were tested for the induction of lysogenie bacteria to liberate phages in E. coli K12 W1177 and W1485 his~- system. The chemicals used included those known to be mutagenic, tumoricidal, carcinogenic, anti-radiation and/or anti-metabolite.There were found 15 chemicals that gave about 5% induction (about 25 times spontaneous liberation), and 3 chemicals that gave at least 45% induction. Among these effective chemicals, at least 60% were known to be mutagenic agents. Of the 6 chemicals that were found to be inhibitory to the spontaneous liberation of phages, 4 were uracil analogues.The results are briefly discussed in connection with the relationship between the bactericidal and the induction potency of these chemicals, and with the possible mechanism of the action of such base analogues as 5-bromodeoxyuridine, diazouracil and 2-thiouracil.更多还原