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亚州飞蝗(Locusta migratoria Меуеn)消化道中苯基硫酸酯酶的活性
THE PHENYLSULFATASE ACTIVITY OF THE DIGESTIVE TRACT OF THE LOCUST(Locusta migratoria Meyen)
【摘要】 <正> 飞蝗是我国农业上主要害虫之一,测定其消化道中的某些酶的活性对於抗蝗药剂的设计、合成和应用可以提供一些有用数据。一.实验方法取飞蝗的前肠及中肠,将它们分别作成丙酮粉,再用此粉的甘油提取液与硫酸苯基钾作用,然后以此色法观察其苯基硫酸酯酶的活性。
【Abstract】 Acetone powders were prepared from chosen parts of the digestive tract of the locust.Diluted glycerol extracts of the powders were allowed to react with potassium phenylsulfate buffered at pH 6.2 under specific conditions for one hour.The phenol liberated from the substrate was determined qualitatively and quantitatively by colorimetry according to the method of L.D. Abbott.Positive results were obtained. The phenylsulfatase activity was expressed as the ratio between the amount of the phenol liberated in milligrams and the volume of the enzyme solution taken in milliliters.
- 【文献出处】 北京师范大学学报(自然科学版) ,Journal of Beijing Normal University(Natural Science) , 编辑部邮箱 ,1957年02期
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