【摘要】 1.应用组织化学的方法研究了从小白鼠肠系膜静脉内取出的日本血吸虫成虫体内核酸、氨基酸、糖元和磷酸酶的分布情况。 2.成虫体内核酸的分布虽较广泛,但以雄虫的睾丸、雌虫的卵巢和卵黄腺中的含量为最高。在睾丸中主要为DNA,而卵巢和卵黄腺中则为RNA。 3.虫体的肌纤维、卵巢和睾丸中的生殖细胞核、卵黄细胞和肠管上皮细胞对显示酪氨酸、色氨酸和组氨酸的偶联重氮反应呈现阳性。角皮下肌层、实质组织细胞和卵黄细胞的颗粒滴呈强的溴酚蓝阳性反应。而对铁氰化物的反应仅卵黄细胞的颗粒滴呈现阳性。 4.糖元主要分布于虫体的实质组织和各种肌纤维内,尤以雄虫抱雌沟附近的实质组织内含量最多。在生殖器官中,仅成熟的卵细胞胞浆含有少许。 5.成虫体表角皮含有大量的碱性磷酸酶,而卵模上皮细胞、卵黄细胞和排泄管壁亦呈阳性反应。酸性磷酸酶主要分布于虫体的角皮下肌层、实质组织细胞核、肠管上皮细胞、雌雄生殖细胞和卵黄细胞内。 6.对于日本血吸虫体内各器官组织中所含的上述各种化学物质的生理意义进行了讨论,并认为血吸虫除肠道摄食外,尚可通过体表角皮吸收碳水化合物等营养物质。更多还原

【Abstract】 Adult Scfiistosoma japonicum worms obtained from the mesenteric veins of artificially infected mice were studied histochemically for the distribution of nucleic acids, amino acids, glycogen and phosphatases. The results obtained may be summarized below.1. Nucleic acids were seen mostly in the parenchyma cells and reproductive organs. In the testes, DNA was very rich, while RNA was found abundantly in the vitcllaria and ovary.2. The positive reaction for tyrosine, tryptophan and histidine was found in the subcuticular muscles, ovary, testes, vitelline cells and the intestinal epithelium. The bromo-pheriol-blue test for basic proteins was strongly positive in the subcuticular muscles, parenchyma cells and vitellaria, while the positive reaction for ferricyanide method was found only in the cytoplasm of the vitelline cells. 3. Glycogen was richly distributed in. the parenchyma, especially in the region adjacent to the gynaccophoric canal of male worms. It was also present, though in small quantity, in the mature ovum.4. Alkaline phosphatase activity was most pronounced in the Cuticular layer and the epithelial cells of ootypc, while moderately positive reaction was observed in the excretory canal and vitelline cells. Acid phosphatase activity was shown most strongly in the subcuticular muscle layer and the parenchyma cells; the epithelia of the intestinal canal and the sexual glands showed also positive reaction.5. The role played by the substances demonstrated histochcmically in various organs of this fluke was discussed. It is suggested that the phosphatase activity in the cuticular layer might be associated with the active absorption of carbohydrates.更多还原