【作者】 张斌；

【导师】 贺红；

【作者基本信息】 武汉大学 ， 口腔正畸学（专业学位）， 2018， 博士

【摘要】 目的:本文通过比较分析慢性牙周炎(CP)、顽固性牙周炎(RP)的血液和唾液组织中基因表达谱,筛选不同类型牙周炎在不同组织中具有特征代表性的基因,为区分和治疗不同类型牙周炎提供帮助。材料与方法:从GEO数据库中下载GSE43525数据,共23个患者血液和唾液组织表达谱样本:13个样本来自血液(包括4个正常人,4个CP,5个RP),10个样本来自唾液(包括3个正常人,4个CP,3个RP)。我们首先对每种类型的表达谱样本进行了样本描述比较,用limma软件包筛选两种牙周炎分别在血液和唾液中的差异表达基因,再对各组中筛选得到的差异表达基因进行层次聚类分析,然后分别用DAVID和KOBAS软件包进行功能富集及通路分析,最后借助WebGestalt方法,搜索得到显著相关的miRNA。结果:CP样本得到213个差异表达基因(DEGs),RP样本得到45个DEGs。功能富集分析显示CP患者血液和唾液的DEGs主要分别富集于与翻译相关生物学过程及细胞凋亡调控的通路上;而RP患者血液和唾液的DEGs主要分别富集在免疫应答的生物学过程及对有机物和细菌感染反应的通路上。搜索得到了一些与CP相关的miRNA如miR-381及miR-494,其对应的靶基因为CD24,EST1,MTSS1,ING3,CCND2 及 SYNE2。结论:本文为从免疫角度研究不同种类的牙周炎提供了向导,筛选得到的特征基因以及miRNA为识别和靶向治疗牙周炎提供了依据。更多还原

【Abstract】 Objective:This study aimed to identify characteristic representative genes through a comparative analysis of gene expression profiles in the blood and saliva of chronic periodontitis(CP)and refractory periodontitis(RP)patients to provide new treatment strategies that may be helpful in the treatment of different forms of periodontitis.Materials and Methods:GSE43525 was downloaded from Gene Expression Omnibus.In the dataset,thirteen samples were from blood including 4 controls,4 CP and 5 RP samples,and ten samples were from saliva including 3 controls,4 CP and 3 RP samples.After comparing the CP and RP samples,differentially expressed genes(DEGs)between these two types of periodontitis in the blood and saliva samples were identified by an LIMMA package.Then,functional and pathway enrichment analyses were performed by DAVID and KOBAS,respectively.The significantly associated miRNAs in CP and RP were searched by WebGestalt.Results:In total,213 DEGs in CP and 45 DEGs in RP were identified.Functional enrichment showed that the DEGs of CP were mainly enriched in ribosome and regulation of apoptosis-related pathways in blood as well as saliva,while the DEGs of RP were significantly enriched in immune responses and response to organic substance-related pathways.Several miRNAs,such as miR-381 and miR-494,were identified as being closely associated with CP.In addition,CD24,EST1,MTSS1,ING3,CCND2 and SYNE2 might be potential targets for diagnosis and treatment of CP.Conclusions:The identified DEGs and miRNAs might be potential targets for the treatment of chronic and refractory periodontitis.更多还原