【作者】 张磊；

【导师】 张乃生；

【作者基本信息】 吉林大学 ， 兽医博士（专业学位）， 2017， 博士

【摘要】 犬子宫内膜炎(endometritis)是中老年母犬的的一种重要疾病。当前,犬子宫内膜炎越来越受到宠物主人及医院的重视。本论文对郑州市3个地区4家宠物医院2013-2015年就诊犬的档案资料进行统计,汇总共获得有效个案资料613例,分析统计子宫内膜炎的发生与年龄、发情情况和品种之间的关系。实验结果显示,近几年来,子宫内膜炎有逐年上涨的趋势;从犬种上看,小型犬发病率居多,小型犬里以吉娃娃、博美高发;大型犬中金毛发病率高,从年龄上看,6岁以上的犬高发,尤其是无交配史、无生育史的高发。随后,我们采取病史回顾法对本院五年来确诊的68例子宫内膜炎病例的临床诊断情况进行了分析。结果显示,子宫内膜炎的诊断是一个综合的诊断方法,通过病史,结合症状及实验室检查最终确诊为子宫内膜炎。此外,我们进一步研究了其发病机制。结果表明,孕酮的含量与NF-κB及MAPKs信号通路各分子磷酸化水平呈正相关。孕酮含量的增加会促进犬子宫组织炎症损伤。然而,雌激素含量与作用同孕酮相反。随后,我们选取了临床上诊断为开放型子宫内膜炎的犬,作为研究对象。探究鱼腥草素钠(sodium houttuyfonate,SH)对其治疗效果。结果表明,单独给予SH后,患病犬的临床指标、雌激素和孕酮的含量均被有益调控。联合用药中加入SH后,这些临床指标、雌激素和孕酮的变化较为显著。其有效率为86.7%,大于阳性对照组有效率的73.3%。这些结果表明,SH也许具有治疗开放型犬子宫内膜炎的效果。其与临床常用治疗犬子宫内膜炎的药物(拜有利和缩宫素)合用后效果更为显著。最后,我们通过体外实验初步研究了SH对子宫炎症的抗炎效果及抗炎机制。结果表明,SH能够明显抑制LPS介导的促炎细胞因子的增加,同时能够抑制NF-κB的激活。这些结果暗示:现今我国犬患子宫内膜炎的发病几率逐年升高,子宫内膜炎的诊断是一个综合的诊断方法。其发病机制可能与雌激素、孕酮含量及信号通路的激活有关。SH也许对犬子宫内膜炎的发生具有一定的治疗效果。更多还原

【Abstract】 Canine endometritis is an inflammation in the endometrium of canine which is induced by the external factors.Pet owners and hospitals pay more attention on canine endometritis nowadays.This paper makes some statistics on suffering canines in four pet hospitals of three districts of zhengzhou in 2013-2015.After adding up the whole data,we obtained 613 effective cases,analyzed the relationship among the occurrence of endometritis,ages and breed variety.Our experiment drew a conclusion,endometritis has shown a growing trend for the past few years;As for the breed variety,smale dogs have taken up a great proportion of the morbidity such as Chihuahua,bo mei gao.As for the towser,the Golden Retriever occupies a large proportion of the morbidity;As far as the age is concerned,canines whose age are more than 6 have a high morbidity,especially the ones that have no history of mating or fertility.Whereafter,we dated back to 68 cases of canine endometritis whose diagnosis is definite,and analyzed the clinical diagnosis and treatment.As the result shows,the diagnosis of endometritis is a comprehensive diagnostic method,through the medical history and combined with the symptoms and laboratory tests can make the endometritis clear.Furthermore,we further study the pathogenesis of endometritis.It turned out that there was a positive correlation between the content of progesterone and the molecular phosphorylation level of NF-κB and MAPK.The above results show that the occurrence of canine endometritis is related to the content of progesterone and estrogen.Subsequently,we selected the canines that were clinically diagnosed to be open endometritis as our study object.Probe the effect of SH on its treatment.The results indicated that clinical indicators,estrogen and progesterone levels of suffering canines were all regulated positively after given SH individually.The change of clinical indicators,estrogen and progesterone content was particularly conspicuous after added SH to the drug combination.Its efficiency(86.7%)was higher than the efficiency of the positive control group(73.3%).These results suggest that SH has the effect of treating open endometritis.Its therapeutic effect will beenlarged when combined with the drugs that commonly used to treat canine therapy.Finally,we studied the anti-inflammatory effect and mechanism of SH in vitro experiments.The results indicate that SH can obviously inhibit the increase of LPS mediated proinflammatory cytokines,and suppress the activation of NF-κB pathway.These results suggest that,nowadays,the incidence of endometritis is increasing year by year in domestic,the diagnosis of endometritis is a comprehensive diagnostic method.Its pathogenesis may be related to the estrogen,progesterone levels and the activation of signal transduction pathways in the body.SH may play an important role in the protect effect on the occurrence of canine endometritis.更多还原