益坎胶囊对勃起功能障碍大鼠阴茎海绵体平滑肌细胞表型转化和超微结构的影响

【作者】 于军桥；

【导师】 张蜀武；

【作者基本信息】 成都中医药大学 ， 中医外科学， 2012， 博士

【摘要】 目的：探讨中药益坎胶囊对勃起功能障碍大鼠阴茎海绵体平滑肌细胞表型转化及超微结构的影响,以揭示益坎胶囊治疗勃起功能障碍的机理。方法：选取SD大鼠50只,其中10只设为正常组,另40只通过结扎双髂内动脉造成勃起功能障碍模型。造模后将其中10只设为模型对照组,其余30只灌胃给予益坎胶囊,浓度分别为1.88g/kg、0.94g/kg、0.47g/kg。给药一个月后,经腹腔注射阿朴吗啡,观察大鼠呵欠次数和阴茎勃起次数；切取大鼠的阴茎组织标本,通过免疫组化法观测海绵体平滑肌细胞收缩型性标志物碱性调宁蛋白(calponin1)和合成型标志物骨桥蛋白(OPN)的表达,并在透射电镜下观察海绵体平滑肌细胞超微结构的变化。实验结果与正常组和模型对照组进行比较。结果：注射阿朴吗啡后,模型对照组大鼠勃起次数明显减少,与正常组相比,差异有统计学意义；给药后大鼠勃起次数有明显改善。免疫组化结果显示,模型对照组大鼠海绵体平滑肌细胞calponin1表达减少,而OPN表达增多,与正常组相比差异有统计学意义；与模型对照组相比,给药后大鼠海绵体平滑肌细胞calponin1表达增多,OPN表达减少。电镜下,模型对照组大鼠海绵体平滑肌细胞中线粒体重度肿胀,嵴减少、或消失、甚或出现空泡化等表现；给药后大鼠线粒体肿胀明显改善。结论：益坎胶囊能改善勃起功能障碍大鼠的勃起功能,抑制海绵体平滑肌细胞表型由收缩型向合成型转化,并能改善海绵体平滑肌细胞的线粒体形态结构。更多还原

【Abstract】 Objectives:To observe the effect of yikan capsules on phenotypic transformation of corpus cavernosum smooth muscle cells (CCSMC) and ultra-structure in rats with erectile dysfunction(ED). Methods:50rats were randomly divided equally into5groups, in which10rats were assigned in normal group,40rats were made into erectile dysfunction models by ligating bilateral internal iliac arteries. After ligation,10rats were assigned in control group, and the other30rats were given yikan capsules with doses of1.88g/kg,0.94g/kg,0.47g/kg, respectively. After one month, each rat was injected apomorphine subcutaneously to record the times of yawning and penile erection. Afterwards, expressions of calponin-1and osteopontin(OPN) in CCSMC were examined via immunohistochemical staining, and ultra-structures of CCSMC were observed with transmission electron microscope. Results:After injection of apomorphine, times of penile erection of rats in control group decreased, compared with normal group, suggesting significant difference between two groups. Times of penile erection of rats given yikan capsules increased, compared with control group. Results of immunestochemical staining showed that expressions of calponin-1were decreased, while, expressions of OPN were increased in CCSMC in control group. Whereas, when given yikan capsules, expressions of calponin-1were increased, and expressions of OPN were decreased in CCSMC. Examination with transmission electron microscope showed that there was severe swelling in mitochondrion, mitochondrial crista decreased, or disappeared, even vacuolated in control group. The swelling in mitochondrion has been improved in rats given yikan capsules. Conclusions:It is shown that yikan capsules could improve penile erection of rats with ED, inhibit phenotypic transformation of CCSMC from contractile to synthetic type, and improve morphological structure of mitochondrion in CCSMC.更多还原