【作者】 徐佳；

【导师】 王洪艳； 李俊锋；

【作者基本信息】 吉林大学 ， 应用化学， 2009， 博士

【摘要】 本文以电纺丝技术为依托,研究了组织工程纤维支架材料的制备和性能。利用混合溶剂的比例对电纺聚乳酸纤维直径进行控制,制备出了不同孔径的聚乳酸纤维支架,证明了纤维直径和支架孔径是可控的;研究了不同质量分数的壳聚糖纺丝液的电纺行为,选出了最佳的纺丝液浓度;提出用三氟乙醇和水混合做溶剂,制备了明胶纤维支架;采用戊二醛对壳聚糖和明胶纤维支架进行交联,提高了壳聚糖纤维支架的抗水性;通过拉伸实验测试了各种纤维支架的力学性能;首次用电纺丝技术制备出了壳聚糖/聚乳酸复合纤维支架,这种支架兼备壳聚糖与聚乳酸的优点,是极具潜力的组织工程支架材料;将兔脂肪间质干细胞和兔角膜基质细胞在以上支架上进行了体外培养试验,用激光共聚焦显微镜和扫描电镜观察了细胞在支架上的生长情况,证实细胞不仅可以在材料的表面生长,也可以向材料的内部生长;采用胶体保护法结合电纺丝技术,制备了两种有机-无机掺杂纳米纤维,即含氟明胶纳米纤维及CuS/聚乙烯醇纳米纤维,并研究了其形成机理,实现了有机-无机掺杂纳米纤维支架的制备。更多还原

【Abstract】 Tissue engineering is a new cross-discipline for studying tissue organ and its functional substitute using the principle and method of engineering and life science. The scaffold materials play a central role in tissue engineering research, they not only provide structural supporting for the particular cell, but also play a template role to guide tissue regeneration and control organizational structure. Therefore, it is an important work that looking for a good biocompatibility and biodegradability scaffold material with a large three-dimensional porous.Electrospinning is a simple and effective processing technology by which polymer solution or melt be spun into nanofiber in strong electrical field. The non-woven fabric electrospun has a large surface area and high porosity and can promote cell migration and proliferation. Therefore, electrospinning technology has a wide application prospect in tissue engineering field. In this stsdy, we prepared a series of fiber tissue engineering scaffold using PLLA, chitosan and gelatin base on electrospinning, and characterized their properties.In the second chapter, PLLA was dissolved in the mixed solvent with different proportions of chloroform and DMF, and was electrospun into fibers. It was found that the fiber diameter, the porosity of material and Young’s modulus gradually decrease with the increase of DMF. By culturing ADSCs cell on the material, we found that the cell could grow not only on the surface but also inside of material. In the third chapter, we prepared chitosan nanofibers tissue engineering scaffold using TFA as a solvent. At the mass fraction of 1.77%, the chitosan fiber has the largest diameter, the best morphology and the best apparent of membrane. After crosslinked with GTA vapor, the nanofibers scaffold had a good water resistance and higher break strength. By culturing ADSCs cell on the material, we found that the material had a good cell adhesive ability, and cell could grow inside the material.In the fourth chapter, we succeeded for the first time in preparing chitosan/PLLA blended fibers by an electrospinning technique. We find that trifluoroacetic acid is a suitable solvent for the electrospinning of chitosan/PLLA blended fibers. With the increasing of content of PLLA, the morphology of fibers became finer. By culturing ADSCs cell and RCSC cell on the material, we found that the material had a good cell adhesive ability, and cell could grow inside the material. It was assumed that the production would have a great potential application in the tissue engineering.In the fifth chapter, we prepared gelatin nanofiber tissue engineering scaffold using a mixed solvent of TFE and water. The material had a high porosity. After crosslinked with GTA vapor, the nanofibers scaffold had a good water resistance and good mechanical properties. The material degradation situation was studied. By culturing RCSC cell on the material, we found that the material had a good cell adhesive ability, and cell grew well.In the sixth chapter, fluoride was introduced into gelatin nanofibers by electrospinning process successfully. CaF2 nanoparticles were well dispersed in the gelatin nanofiber matrix. The fluoride-containing gelatin nanofibers scaffold crosslinked with saturated GTA vapor had a good water resistance and higher break strength. Based on similar principle, we also prepared CuS/PVA nanofiber. So, it was an effective method to prepare organic/inorganic nanofiber. This novel material would have a special potential application in the tissue engineering.In conclusion, electrospinning is an effective processing technology to prepare nanofibers scaffold. We believe that we could prepare scaffold according to our will with further understanding electrospinning technique.更多还原