【作者】 陈东红；

【导师】 沈大棱； 明凤；

【作者基本信息】 复旦大学 ， 遗传学， 2007， 博士

【摘要】 被子植物的SQUA家族(AP1／FUL家族)属于MADS-box基因，其在营养到生殖发育的转变中扮演着重要角色。并且这个家族的起源与被子植物花结构的固定同时发生。在这里，我们从蝴蝶兰cDNA中分离出两个MADS-box基因，即ORAP11和ORAP13；他们属于SQUA家族的单子叶FUL-like分支。RT-PCR显示这两个基因在花芽强表达，在营养器官也可检测到。随后在成花中，ORAP11基因仅在蕊柱可检测到，但ORAP13基因在所有的花器官没有信号。原位杂交表明两者在正在发育的花瓣和唇瓣的边缘和尖端，正在发育的蕊柱，和胚珠中有表达。ORAP基因在烟草的过量表达导致提前开花和植株形态构建的变化。我们的结果表明这两个基因在蝴蝶兰中功能冗余，在植物的成花转变过程中和形态构建方面有重要功能。更多还原

【Abstract】 The SQUA family (AP1/FUL family) of MADS-box genes plays an important role in the transition from the vegetative to the reproductive development of angiosperms, and its origin might be concurrent with fixation of floral structure in angiosperms. Here, we isolated two Phalaenopsis MADS-box genes designated ORAP11 and ORAP13, both of which belong to the monocot FUL-like clade of the SQUA family. RT-PCR showed that both genes are strongly expressed in the floral bud, and also detected in the vegetative organs. During later stages, ORAP11 was only detected in the column, but ORAP13 signal was absent from all of the floral organs. In-situ hybridization experiments detected both genes in the tips and margins of developing petals and lips, the developing column, and ovule. Over-expression of both genes in tobacco induced early flowering and changed plant architecture. Our results suggest that in Phalaenopsis, both genes might share partly redundant activities and play important roles in the process of floral transition and morphological architecture.更多还原