【作者】 李莉平；

【导师】 王自能； 曾耀英；

【作者基本信息】 暨南大学 ， 妇产科学， 2006， 博士

【摘要】 第一章 早、中、晚孕期胎盘因子体外抗HIV-1的研究 目的：探讨早、中、晚孕期胎盘因子(PF)体外抗HIV-1的作用及其在HIV-1垂直传播中可能的作用。 方法：采用荧光染料Calcien-AM标记的H9／HIV-1 ⅢB分别与早、中、晚孕期不同稀释浓度的PF作用后，与MT2细胞混合培养，荧光显微镜下观察合胞体的形成；用游离的HIV-1 ⅢB感染MT2细胞，并分别与早、中、晚孕期不同稀释浓度的PF作用后，用MTT法检测HIV-1感染细胞的存活率，用HIV-1 p24抗原试剂盒检测细胞培养上清中p24抗原含量的变化。 结果：各孕期PF并不能抑制MT2和H9／HIV-1 ⅢB细胞的融合，但可以增加HIV-1感染细胞的存活率及减少HIV-1 p24抗原的表达，且效应以早孕期PF最大，中孕期PF其次，晚孕期PF最小，并与剂量呈正相关。 结论：PF在体外具有抗HIV-1的作用，并呈现孕期和剂量相关性，可能在阻断HIV-1垂直传播中具有重要作用。 第二章 人绒毛膜促性腺激素在HIV-1感染中作用的体外研究 目的：探讨PF是否通过人绒毛膜促性腺激素(hCG)具有体外抗HIV-1的作用，以及hCG在HIV-1垂直传播中可能的作用。 方法：采用Calcien-AM标记的H9／HIV-1 ⅢB与不同稀释浓度的hCG作用更多还原

【Abstract】 Chapter 1 Study on the 1st, 2nd and 3rd trimester placental factorsagainst HIV-1 in vitroObjective: To investigate the effects of the 1st, 2nd and 3rd trimester placental factors (PFs) on human immunodeficiency virus type 1 (HIV-1) infection in vitro, and to evaluate their possible roles in HIV-1 intrauterine infection.Methods: Calcein-AM stained H9/HIV-1ⅢB cells were treated with different concentrations of the 1st, 2nd and 3rd trimester PFs respectively, then mixed with MT2 cells, HIV-1-mediated syncytium formation was observed under fluorescent microscopy. MT2 cells were treated with cell-free HIV-1 ⅢB, then washed and cultured with different concentrations of the 1st, 2nd and 3rd trimester PFs respectively, the protecting effect of PFs on HIV-1-infected cells was determined with MTT, viral replication was evaluated by measuring the levels of p24 antigen in culture supernatants with ELISA.Result: PFs did not suppress HIV-1-mediated syncytium formation, but they significantly increased HIV-1-infected cells’ viability and decreased p24 antigen levels. Moreover, their effects were trimester- and concentration-dependent.更多还原