【作者】 韩玉珠；

【导师】 殷涌光；

【作者基本信息】 吉林大学 ， 农业机械化工程， 2006， 博士

【摘要】 我国在中药有效成分提取方法上以传统方法为主,提取时间长、提取过程繁琐、提取出来的有效成分复杂、含量比例较低,急需开发新的方法提取传统的中药资源,增强我国传统中药的优势,加快中医药走向世界的步伐。高压脉冲电场(PEF)杀菌技术已被公认为国际上研究最热门、最先进的灭菌技术之一,是对传统灭菌方法的革命,其创新性、科学性和先进性不容置疑。本文根据PEF技术对微生物细胞的作用机理,首次把PEF技术应用到经济药用动物中国林蛙有效成分多糖的提取、分离上,为中药有效成分的提取提供了一条全新的途径,以便于深入发展和拓宽PEF技术的应用范围,同时满足中药现代化对低能耗、低操作费用、高效的高新技术的要求。经过大量的实验研究,获得了合理的加工工艺,并通过对PEF提取法和常规方法的比较、提取机理的分析、提取动力学模型的建立,证明这种方法是完全可行的,并对该方法的可行性做出了理论解释,建立了一套可以快速提取的工艺方法,为该方法的工业化生产奠定了理论和实践的基础。更多还原

【Abstract】 Pulsed electric field (PEF) non-thermal pasteurization technique was known as the one of the most interesting and advanced technology on international. It is a revolution in traditional pasteurization technique. It is unassailable that it is innovative, scientific and advanced. It is noticeable that PEF technology is a crossed branch of electronics, chemistry, microbiology, physics and engineering technique. Its mechanism research to microorganism inactivation will provide a new method for life sciences question desiderated to resolve, such as cell components extraction, cell amalgamation et al. But there isn’t any research in this way. So it is necessary to develop the PEF technology to meet the market’s need have low consumed and high effect to a new technique.The extraction, separation and purification are the key basic technologies of Chinese drug modernization. Because the essential components or the cluster of essential components must be attained, the modernization of Chinese drug may be carried out. Nowadays, the traditional extraction methods give priority to the Chinese drug modernization, which are low effect, long time low extraction quantity. So it is in dire need of a new method to quicken the Chinese drug’s step to the world.We come to a conclusion on the PEF as follows: extracting at normal temperature, the essential components without loss and denaturalization, time of extraction being short, the achievable yield being high, purification and separation being easy, economizing on energy and without contamination. PEF is better than the traditional technologies The PEF technology is firstly used to extract Rana temporaria chensinensis David’s polysaccharide in this paper. Several key technical problems of the technology are studied as follows:1. PEF technology is used to extract Rana temporaria chensinensis David’s polysaccharide first time. This is a new exploration and probing to extract the essential components from Chinese herbs. The affect factors of PEF are analyzed with the extraction of Rana temporaria chensinensis David’s polysaccharide in detail. The basic parameters’ranges of PEF to extract the Rana temporaria chensinensis David’s polysaccharide are as following: electric field intensity 20kV/cm, pulse number 6, extraction liquid 0.5%KOH. These results not only provide experimental and theoretical basis more for extracting Rana temporaria更多还原