【作者】 孙钢；

【导师】 李龙江；

【作者基本信息】 四川大学 ， 口腔临床医学， 2006， 博士

【摘要】 目的：成釉细胞瘤是最常见的牙源性肿瘤，好发于下颌骨，具有浸润性生长特性，主要来源于牙胚发育中未退化的牙源性上皮，目前对其发生发展机制还不清楚。现有研究结果表明，基因调控与成釉细胞瘤发生发展密切相关，针对其主要调控基因进行研究，有望在理论认识上产生突破。同源异型盒基因是调节胚胎发育的主控基因，在牙胚发育中起关键作用，本课题旨在探索同源异型盒基因对成釉细胞瘤发生发展的影响，以期发现有价值的基因，拓宽对该肿瘤的认识，同时利用维甲酸进行诱导分化治疗，为深入认识该疾病的发生发展机制及治疗探索，提供新的思路和实验依据。 方法：设计含同源异型盒基因家族的oligo芯片，筛选出成釉细胞瘤较帽状期／钟状期牙胚中差异表达基因。针对候选基因设计引物，在14例临床标本和3例牙胚组织中验证表达差异结果。原代培养成釉细胞瘤细胞，观察其形态及生长情况，作特异性抗体检测。用全反式维甲酸诱导肿瘤细胞分化，采用光学显微镜、透射电镜、MTT、流式细胞仪、免疫组化，检测肿瘤细胞形态，增殖指数，生长抑制率，细胞周期，凋亡指数的变化，最后应用实时荧光定量PCR检测目的基因的表达变化。 结果：基因芯片筛选出73个差异表达基因，其中上调基因11个，下调更多还原

【Abstract】 Objectives: Ameloblastoma(AM) is the most frequent odontogenic tumor. It has local infiltrating growth potential and occurs most frequently in the molar region of the mandible. According to retrospective researches, ameloblastoma is believed to arise from the enamel organ, the remnant of the dental lamina and the odontogenic epithelium. The differentiated level of AM cells remains at the cap/bell stage of tooth germ development. However, the genetic mechanism about the development and occurrence of ameloblastoma is poorly understanded yet. Till now, the main therapy of AM is extensive resection in order to reduce the recurrence. Homeobox gene is a family of regulatory genes encoding transcription factors, which primarily play a crucial role in embryonic development and the same contribution in the tooth germs. The aim of this study is to explore the affection of homeobox genes in progression and occurrence of the ameloblastoma. We expect to find valuable genes that may be involved in the course, and use trans retinoic acid to induce difference. We hope our finding could provide a new basis for further studies intending to improve the understanding of the molecular pathway of ameloblastoma. Methods: We designed oligo gene chip with homeobox gene to screen更多还原