【作者】 张庆鸿；

【导师】 梁星；

【作者基本信息】 四川大学 ， 口腔临床医学， 2006， 博士

【摘要】 口腔科学中，缺牙修复，正畸牙移动，牙周重建及拔牙创的愈合等均涉及到骨代谢。颌骨处在一个不断动态改建的过程中，骨重建不但包括成骨细胞的新骨形成，同时还包括破骨细胞的旧骨吸收。当破骨细胞数量增多、活性增强，将会导致骨吸收增加，往往会出现牙根吸收松动等方面的疾病；反之，破骨细胞数量减少、活性降低又会导致骨吸收减慢，通常会出现牙萌出障碍等方面的疾病。在骨吸收过程中，破骨细胞被各种刺激因素(主要是应力)激活后，胞内的空泡型质子泵通过转运H~+而达到骨基质的酸破坏，从而形成骨吸收陷窝。由此可见，破骨细胞空泡型质子泵在骨改建过程中具有重要的作用。然而，由于破骨细胞是一种终末分化的细胞，存在培养、检测、存活时间短、细胞数量少及无细胞株等局限性，大大限制了破骨细胞的研究。目前尚未检索到流体剪切力对破骨细胞空泡型质子泵mRNA影响的研究报道。 [目的]本研究旨在对鼠破骨细胞的体外培养鉴定和流体剪切力作用下细胞形态学及空泡型质子泵mRNA的变化作一探讨。 [方法]本研究通过动物长骨机械分离法获得破骨细胞，进行培养鉴定后，对破骨细胞施加剪切应力，观察细胞形态学变化，并用实时荧光精确定量聚合酶链式反应检测空泡型质子泵mRNA的表达水平。更多还原

【Abstract】 In stomatology, many cure and prevention processes involve bone metabolism. Jaw suffers from a dynamic bone remodelling, which includes not only bone formation affected by osteoblasts, but also bone resorption by osteoclasts. When osteoclast increases amount or activity, resulting in more bone resorption, otherwise, more bone formation. These changes would create tooth loose or difficulty in tooth eruption. In course of osteoclastic resorption, vacuolar proton pump in osteoclasts activated by various stimulating factors, mainly stresses, transports hydrogen ion to acidify bone matrix, forming Howship’s lacuna finally. Consequeltly, vacuolar proton pump in osteoclasts takes important effect in bone remodelling. However, osteoclast is a kind of terminally differentiated cell with shortcomings, such as difficulty in culture, short survival time, less amount and no cell strain and so on, which mostly restrain study in osteoclasts. At present, there are not articles about effect of flow shear stress on vacuolar proton pump mRNA in osteoclasts.[Aim] The present study investigated effect of flow shear stress on morphology and vacuolar proton pump mRNA in rat osteoclasts.更多还原